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626 ORGANS OF GENERATION.
Mitjukoff l
has isolated and investigated a colloid from an ovarial cyst. It
had the following composition: C 51.76, H 7.76, N 10.7 S 1.09, and 28.69 per
cent, and differed from mucin and pseudomucin by reducing Fehling’s solu-
tion before boiling with acid. It must be remarked that pseudomucin, on boiling
sufficiently long with alkali, or by the use of a concentrated solution of caustic
alkali, also splits and causes a reduction. This reduction is nevertheless weak
as compared with that produced after boiling with an acid. The body isolated
by Mitjukoff is called paramucin.
The pseudomucin as well as colloid are mucoid substances, and the
carbohydrate obtained from them is glucosamine (chitosairine), as espe-
cially shown by Fr. Muller, Netjberg and Heymann.2
From pseudo-
mucin Zangerle 3
obtained 30 per cent glucosamine, and Netjberg and
Heymann have shown that the glucosamine is the only carbohydrate
regularly taking part in the structure of these substances. Still there are
reports as to the occurrence of chondroitin-sulphuric acid (or an allied
acid) in pseudomucin or colloid (Panzer), but this is not constant
according to the experience of Hammarsten.
As hydrolytic cleavage products of pseudomucin Otori obtained,
besides carbohydrate derivatives such as levulinic acid and humus sub-
stances, leucine, tyrosine, glycocoll, aspartic acid, glutamic acid, valeric
acid, arginine, lysine, and guanidine. The quantity of guanidine, it
seems, was greater than that which could be derived from the arginine,
hence this body probably originated from another complex. Pregl 4
obtained on the hydrolysis of a colloid, which behaved like paramucin,
no glycocoll and only traces of diamino acids, but otherwise the same
amino-acids as Otori found, besides alanine, proline, phenylalanine and
tryptophane.
The detection of metalbumin and paralbumin is naturally connected
with the detection of pseudomucin. A typical ovarial fluid containing
pseudomucin is, as a rule, sufficiently characterized by its physical proper-
ties, and a special chemical investigation is necessary only in cases where a
serous fluid contains very small amounts of pseudomucin. The pro-
cedure is as follows: The protein is removed by heating to boiling with
the addition of acetic acid; the filtrate is strongly concentrated and pre-
cipitated by alcohol. The precipitate, a transformation product of
pseudomucin, is carefully washed, with alcohol and then dissolved in water.
A part of this solution is digested with saliva at the temperature of the
body and then tested for glucose (derived from glycogen or dextrin).
If glycogen is present, it will be converted into glucose by the saliva;
precipitate again with alcohol and then proceed as in the absence of
1
K. Mitjukoff, Arch. f. Gynakol., 49.
2
Muller, Verh. d. Naturf. Gesellsch. in Basel. 12, part 2; Neuberg and Heymaim;
Hofmeister’s BeitriiKe, 2. See also Leathes, Arch. f. exp. Path. u. Pharm., 43.
1
Mfinch. med. Wochenschr., 1900.
4
Otori, Zeitschr. f. physiol. Chem., 42 and 43; Pregl, ibid., 58.
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