Full resolution (JPEG) - On this page / på denna sida - V. The Blood - I. Blood-plasma and Blood-serum - The Blood-plasma
<< prev. page << föreg. sida << >> nästa sida >> next page >>
Below is the raw OCR text
from the above scanned image.
Do you see an error? Proofread the page now!
Här nedan syns maskintolkade texten från faksimilbilden ovan.
Ser du något fel? Korrekturläs sidan nu!
This page has never been proofread. / Denna sida har aldrig korrekturlästs.
FIBRIN. 255
small, less elastic, and not particularly fibrous, lumps. The typical
fibrous and elastic white fibrin, after washing, stands, in regard to its
solubility, close to the coagulated proteins. It is insoluble in water,
alcohol, or ether. It expands in hydrochloric acid of 1 p. m., as also in
caustic potash or soda of 1 p. m., to a gelatinous mass, which dissolves at
the ordinary temperature only after several days; but at the temperature
of the body it dissolves more readily, although still slowly. Fibrin may
be dissolved by dilute salt solutions, after a long time, at the ordinary
temperature, or much more readily at 40° C, and this solution takes place,
according to Arthus and Hubert and also Dastre, 1
without the aid
of micro-organisms. This action is due to proteolytic enzymes car-
ried down by the fibrin or enclosed within the leucocytes (Rulot 2
).
According to Green and Dastre 3
two globulins are formed in the solu-
tion of fibrin in neutral salt solution, and according to Rulot also pro-
teoses (and peptones) on the solution of fibrin containing leucocytes.
Fibrin, like fibrinogen, decomposes hydrogen peroxide, due to a con-
tamination with catalases, but this property is destroyed by heating or
by the action of alcohol.
What has been said of the solubility of fibrin relates only to the typical
fibrin obtained from the arterial blood of oxen or man by whipping
and washing first with water and with common salt solution, and then
with water again. The blood of various kinds of animals yields fibrin
with somewhat different properties, and according to Fermi 4
pig-fibrin
dissolves much more readily than ox-fibrin in hydrochloric acid of 5 p. m.
Fibrins of varying purity or originating from blood from different parts
of the body have unlike solubilities.
The fibrin obtained by beating the blood, and purified as above
described, is alwaj’S contaminated by secluded blood-corpuscles or
remains thereof, and also by lymphoid cells. It can be obtained pure
only from filtered plasma or filtered transudates. For the preparation
of pure fibrin, as well as for the quantitative estimation of it, the spon-
taneously coagulating liquid is at once, or the non-spontaneously coagu-
lating liquid only after the addition of blood-serum or fibrin ferment,
thoroughly beaten with a whale-bone, and the separated coagulum is
washed first in water and then with a 5-per cent common salt solution,
and again with water, and finally extracted with alcohol and ether. If
the fibrin is allowed to stand for some time in contact with the blood
from which it was formed, it partly dissolves (fibrinolysis—Dastre.5
).
This fibrinolysis must be prevented in the exact quantitative estimation
Arthus and Hubert, Arch, de Physiol. (5) 5; Dastre, ibid., (5) 7.
2
Arch, intern, de Physiol., 1.
3
Green, Journ. of Physiol., 8; Dastre, 1. c.
4
ZeiHcl.r. f. Biologie, 28.
5
Archives, de Physiol. (5), 5 and 6.
<< prev. page << föreg. sida << >> nästa sida >> next page >>
